has role has_role has role gene as virulence factor in pathogen gene mutant attenuated in host cell gene mutant attenuated in host has role has_role has role macrophage macrophage 巨噬细胞 Mus musculus Mus musculus 小家鼠 Brucella suis 1330 protein-coding gene of Brucella suis 1330 pheA 1165694 20140405 204722 BR0037 Bin Zhao, Oliver He I NCBI-supplied PMID: 12271122 WEB: http://www.ncbi.nlm.nih.gov/gene prephenate dehydratase protein-coding MUTATION: The product of pheA gene is specifically dedicated to the biosynthesis of phenylalanine. The B. abortus pheA mutant with mini-Tn5 disruption displays nutritional defects in vitro. Experimental findings with the B abortus ilvD, trpB, and pheA mutants suggest that tryptophan and phenylalanine are available to the brucellae in their intracellular niche but that other amino acids (eg, leucine, isoleucine, or valine) are not. The pheA::miniTn5 mutant displayed attenuation in macrophages but not in mice [PMID:15271960]. 1024 virulence factor gene role