has role has_role has role gene as virulence factor in pathogen gene mutant attenuated in host cell line cell gene mutant attenuated in host cell gene mutant attenuated in host has role has_role has role HeLa cell HeLa cell HeLa 细胞 macrophage macrophage 巨噬细胞 Mus musculus Mus musculus 小家鼠 Brucella melitensis bv. 1 str. 16M Bos taurus protein-coding gene of Brucella melitensis bv. 1 str. 16M BMEII0158 1197929 20140222 224914 BMEII0158 Bin Zhao, Oliver He II NCBI-supplied PMID: 11756688; 12271122 WEB: http://www.ncbi.nlm.nih.gov/gene protein-coding two component response regulator MUTATION: FtcR is required in B melitensis 16M for the transcription of the fliF gene during vegetative and intracellular growth, and for the production of the two structural flagellar components FlgE and FliC during vegetative growth. A ftcR mutant has the same virulence phenotype as previously found with structural flagellar mutants. In HeLa cells and bovine macrophages, no attenuation of the ftcR mutant was observed compared to the WT parental strain. In BALB/c mice, the ftcR mutant was not attenuated after 1 week of infection but was attenuated after 4 weeks of infection. FtcR acts as a flagellar master regulator in B melitensis and perhaps in other related alpha-proteobacteria [PMID:17056750]. 1350 virulence factor gene role