has role has_role has role gene as virulence factor in pathogen gene mutant attenuated in host has role has_role has role Mus musculus Mus musculus 小家鼠 Hantaan virus protein-coding gene of Hantaan virus HTNVsMgp1 11599 20140301 2943079 Bin Zhao, Oliver He HTNVsMgp1 NCBI-supplied Other designations: hypothetical protein PMID: 3103329; 17267477; 17342054; 19143481; 19767836 WEB: http://www.ncbi.nlm.nih.gov/gene precursor structural polyprotein protein-coding MUTATION: Two Hantaan virus strains, clone 1 (cl-1), which is virulent in newborn mice, and its attenuated mutant (mu11E10), were used to examine the pathogenesis of Hantaan virus infection in a mouse model and identify virus factors relating to virulence. After subcutaneous inoculation of newborn BALB/c mice, cl-1 caused fatal disease with high viral multiplication in peripheral organs, but mu11E10 produced nonfatal infection with a low level of virus multiplication. Sequence comparison detected a single deduced amino acid change (cl-1 Ile to mu11E10 Thr) at amino acid number 515 in glycoprotein G1. In mouse brain microvascular endothelial cells in vitro, viruses possessing a cl-1-derived M segment grew more rapidly than viruses containing a mu11E10-derived M segment. These results suggest that the single amino acid change in the glycoprotein alters peripheral growth, which affects invasion of the central nervous system in mice [PMID:10982372]. 2877 virulence factor gene role