realizes has_specified_input inheres in function PCR product ABI 377 automated sequencer chain termination sequencing assay A DNA sequencing assay which rely on the use of dideoxynucleotides used in 4 distinct sequencing reaction on the same DNA sample. The dideoxynucleotides, once incorporated in the complementary DNA strand being synthesized by the DNA polymerase prevent any further chain elongation. The newly generated sequences are resolved on a polyacrylamide gel using electrophoresis and labels (either fluorochrome or radioactivity) are used to determine the nucleotide present at a given position PMID: 271968. DNA sequencing with chain-terminating inhibitors. Philippe Rocca-Serra Sanger sequencing dye terminator sequencing url:https://en.wikipedia.org/wiki/Sanger_sequencing chain termination sequencing assay Li-Cor 4300 DNA Analysis System DNA sequencing by synthesis assay metadata complete