has_specified_output has participant scalar measurement datum human antithrombin-III in blood assay antithrombin-III berichrome assay 1 AT-III assay An antithrombin-III (AT-III) assay in which exogenous bovine thrombin and heparin are added to test plasma to form a thrombin-heparin-AT complex. The residual thrombin not bound then hydrolyzes the p-nitroalanine substrate to produce a yellow color, which is read at 405 nm. The intensity of color produced is inversely proportional to the AT present. A calibration is done with standard human plasma reagent and results for a given specimen are reported as a percentage relative to the standard url:http://www.clinchem.org/cgi/content/full/43/9/1783@2009/08/06 antithrombin-III berichrome assay Berichrom(r) Antithrombin III (A) Kit metadata complete