has part realizes is about has_specified_input has_specified_output role of has role gene expression information content entity evaluant role reverse transcribed polymerase chain reaction RNA extract reverse transcription polymerase chain reaction assay An assay that determines the concentration of RNA in a sample in which an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional or real-time PCR. Chris Stoeckert Jie Zheng Penn Group reverse transcription polymerase chain reaction assay polymerase chain reaction assay metadata complete