has role deoxyribonucleic acid reagent role reagent forward PCR primer 5' primer A short oligonucleotide complementary to target DNA (5'->3' on plus strand) that acts as the leader for DNA extension in a PCR reaction. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand. A primer is characterized by its 'melting temperature' (Tm) and pairs of primers should have similar Tm. Philippe Rocca-Serra SP6 promoter, forward primer (SP6 TTTAGGTGACACTATAG) adapted from http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/Glossary.shtml#p forward PCR primer forward primer sense primer ready for release