polymerase chain reaction nested polymerase chain reaction nested-PCR A polymerase chain reaction with modification intended to reduce non-specific binding in products due to the amplification of unexpected primer binding sites. The nested polymerase chain reaction (PCR) involves the use of two primer sets and two successive PCR reactions. The first set of primers are designed to anneal to sequences upstream from the second set of primers and are used in an initial PCR reaction. Jie Zheng VEuPathDB https://en.wikipedia.org/wiki/Nested_polymerase_chain_reaction https://github.com/obi-ontology/obi/issues/1117 nested PCR nested polymerase chain reaction metadata complete