is_specified_output_of has grain primary cultured cell cultured cell population primary cultured cell population 0 A cultured cell population comprised of cells expanded directly from living tissue prior to being passaged. PERSON:Matthew Brush PERSON:Matthew Brush Spleen cells put directly into culture; Cold storage of biopsies from wild endangered native Chilean species in field conditions and subsequent isolation of primary culture cell lines. In Vitro Cell Dev Biol Anim. 2008 Jul 2. PMID: 18594934 cells that have been isolated from some organismal source, expanded in culture, but not undergone a complete passaging (ie are still in culture, or have been lifted from culture and frozen in aliquots for future use) primary cell culture primary cell culture sample primary cultured cell population 2013-6-5 MHB: This OBI class was formerly called 'primary cell culture', but label changed and definition updated following CLO alignment work. Previous definition: 'a primary cell culture is a cell culture where the cells derive from a fresh tissue source.' cell culture splitting metadata complete