realizes has specified input characteristic of function PCR product ABI 377 automated sequencer chain termination sequencing assay dye terminator sequencing PMID: 271968. DNA sequencing with chain-terminating inhibitors. chain termination sequencing assay url:https://en.wikipedia.org/wiki/Sanger_sequencing A DNA sequencing assay which rely on the use of dideoxynucleotides used in 4 distinct sequencing reaction on the same DNA sample. The dideoxynucleotides, once incorporated in the complementary DNA strand being synthesized by the DNA polymerase prevent any further chain elongation. The newly generated sequences are resolved on a polyacrylamide gel using electrophoresis and labels (either fluorochrome or radioactivity) are used to determine the nucleotide present at a given position Sanger sequencing Philippe Rocca-Serra Li-Cor 4300 DNA Analysis System DNA sequencing by synthesis assay metadata complete