has part realizes is about has specified input has specified output achieves_planned_objective characteristic of function gene expression transcription profiling assay reverse transcribed polymerase chain reaction RNA extract sequence data transcription profiling identification objective cap analysis of gene expression assay CAGE PMID:14663149 cap analysis of gene expression assay Philippe Rocca-Serra Marcus Chibucos 5' end-centered expression profiling using cap-analysis gene expression and next-generation sequencing. PMID:22362160 A transcription profiling assay which measures RNA transcript abundances in biological samples by extracting 5' ends of capped transcripts, RTPCR and sequence those. Copy numbers of CAGE tags provide a way of quantification and provide a measure of expression of the transcriptome polymerase chain reaction assay DNA sequencer sequencing assay ready for release