is about has specified input has specified output achieves_planned_objective sequence-specific DNA binding data item genetically modified material protein and DNA interaction identification objective yeast one-hybrid assay bacterial one-hybrid assay bacterial one-hybrid assay Marcus Chibucos Philippe Rocca-Serra A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. PMID:18332042 An assay that identifies the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences, is cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence url:http://en.wikipedia.org/wiki/Bacterial_one-hybrid_system B1H ready for release