has part realizes has specified input achieves_planned_objective role of has role evaluant role polymerase chain reaction DNA extract insecticide resistance detection objective polymerase chain reaction assay insecticide resistance by monitoring known mutations assay insecticide resistance by primer introduced restriction analysis PCR assay VEuPathDB Primer Introduced Restriction Analysis PCR https://github.com/obi-ontology/obi/issues/1117 insecticide resistance by primer introduced restriction analysis PCR assay PIRA-PCR An insecticide resistance by monitoring known mutations assay that detects Single Nucleotide Polymorphisms (SNPs) using PCR technique and mismatched primers that is close to the mutation of interest and digested the PCR productions using restriction enzymes. IRO:0000007 polymerase chain reaction-primer-introduced restriction analysis VEuPathDB PMID: 8339229 specific enzymatic cleavage metadata complete