directed mutagenesis targeted mutagenesis Equivalent to FlyBase term "gene targeting by homologous recombination" (FBcv:0000710) in the origin of mutation branch. gene targeting by homologous recombination url:https://pubmed.ncbi.nlm.nih.gov/23996268/ targeted mutagenesis A directed mutation in which a construct is used to introduce a mutation into a specific genome location through homologous recombination. Susan Bello ORCID:orcid.org/0000-0003-4606-0597 To disrupt the mdr1a gene a 1.6 kb NheI genomic fragment containing exons 6 and 7 was replaced by a hygromycin phosphotransferase cassette in a targeting vector. PMID:7910522 metadata complete