realizes has specified input has specified output characteristic of role of has role function evaluant role DNA sequencing assay DNA extract DNA sequence data DNA sequencer pooled clone sequencing assay Bonita Lam https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049660/ Although a similar pooled clone sequencing method has recently been described by others for metagenome-derived medium-insert plasmids and large-insert fosmids, to our knowledge, we are the first to report the pooled strategy for sequencing of large-insert metagenomic clones while also critically evaluating the performance of this pooled strategy by comparing the results to barcoded reference sequences of the same clones. Dan Berrios, ORCID: 0000-0003-4312-9552 https://github.com/obi-ontology/obi/issues/1106 pooled clone sequencing assay A DNA sequencing assay in which the DNA input is derived from pooled clones (for example BACs and Fosmids) is sequenced. metadata complete