exemplar data of Cluster L2/3 IT primary motor cortex glutamatergic neuron (Mmus) RNAseq 058-069 - CS202002013_182 RNAseq 058-060 - CS202002013_183 CS202002013_183 Zizhen Yao L2/3 IT {"cell_set_preferred_alias": "L2/3 IT", "original_label": "n67", "cell_set_label": "RNAseq 058-060", "cell_set_accession": "CS202002013_183", "cell_set_aligned_alias": "L2/3 IT", "cell_set_additional_aliases": "L2/3 IT", "cell_set_alias_assignee": "Zizhen Yao", "cell_set_alias_citation": "PMID:34616066", "cell_set_structure": "primary motor cortex", "cell_set_ontology_tag": "UBERON:0001384", "species": "Mouse", "modality": "RNAseq", "taxonomy_id": "CCN202002013", "taxonomy_description": "Mouse MOp BICCN taxonomy using multiple RNA-Seq datasets ", "child_cell_set_accessions": "CS202002013_58|CS202002013_59|CS202002013_60", "cell_type_card": "Subclass", "cell_set_color": "#C4EC04"} L2/3 IT PMID:34616066 L2/3 IT L2/3 IT is: Layer 2/3 intratelencephalic-projecting. In Mouse primary motor cortex (CCN202002013), L2/3 IT has 3 child types: L2/3 IT_1, L2/3 IT_2 and L2/3 IT_3. The minimal set of markers required to distinguish this cell type from other cell types in the primary motor cortex is Calb1 and Rasgrf2 (F-beta = 0.842), based on the NS Forest method of marker gene selection. L2/3 IT cells represent 20.93% of all Glutamatergic cells collected in the snRNA-seq 10X v3 B dataset. L2/3 IT cells have an average of 5231 genes detected, which is 118 more than the average for all Glutamatergic cells (5113). The average normalized cortical depth of L2/3 IT cells is 0.204, compared to an average of 0.527 for all Glutamatergic cells. L2/3 IT cells have an average firing rate of 17.34 Hz, compared to 26.59 Hz for all Glutamatergic cells recorded. Other distinguishing features include an input resistance of 91.42 MOhms (156.52 MOhms in all Glutamatergic cells), and upstroke:downstroke ratio of 4.49 (4.03 for all Glutamatergic cells). The most distinguishing morpholgical features of L2/3 IT, as determined by reconstructions from Patch-seq data, are normalized cortical depth (0.17 vs. 0.54 average for all Glutamatergic cells), dendrite total length (4647.41 vs. 4488.4 microns for all Glutamatergic cells), and stems (7.63 vs. 7.25 for all Glutamatergic cells). Subclass L2/3 IT RNAseq 058-060 L2/3 IT n67