exemplar data of Cluster L4 primary motor cortex glutamatergic neuron (Mmus) RNAseq 061-062 - CS202002013_188 PMID:34616066 n72 {"cell_set_preferred_alias": "L4", "original_label": "n72", "cell_set_label": "RNAseq 061-062", "cell_set_accession": "CS202002013_188", "cell_set_aligned_alias": "L4/5 IT", "cell_set_additional_aliases": "", "cell_set_alias_assignee": "Zizhen Yao", "cell_set_alias_citation": "PMID:34616066", "cell_set_structure": "primary motor cortex", "cell_set_ontology_tag": "UBERON:0001384", "species": "Mouse", "modality": "RNAseq", "taxonomy_id": "CCN202002013", "taxonomy_description": "Mouse MOp BICCN taxonomy using multiple RNA-Seq datasets ", "child_cell_set_accessions": "CS202002013_61|CS202002013_62", "cell_type_card": "Subclass", "cell_set_color": "#09CCC6"} L4/5 IT is: Layer 4/5 intratelencephalic-projecting. In Mouse primary motor cortex (CCN202002013), L4/5 IT has 2 child types: L4/5 IT_1 and L4/5 IT_2. The minimal set of markers required to distinguish this cell type from other cell types in the primary motor cortex is Rorb, A830036E02Rik and Kcnh5 (F-beta = NA), per differential expression analysis based on proportion of cells expressing these genes. L4/5 IT cells represent 29.06% of all Glutamatergic cells collected in the snRNA-seq 10X v3 B dataset. L4/5 IT cells have an average of 4676 genes detected, which is 437 less than the average for all Glutamatergic cells (5113). The average normalized cortical depth of L4/5 IT cells is 0.388, compared to an average of 0.527 for all Glutamatergic cells. L4/5 IT cells have an average firing rate of 26.01 Hz, compared to 26.59 Hz for all Glutamatergic cells recorded. Other distinguishing features include an input resistance of 170.7 MOhms (156.52 MOhms in all Glutamatergic cells), and upstroke:downstroke ratio of 4.38 (4.03 for all Glutamatergic cells). The most distinguishing morpholgical features of L4/5 IT, as determined by reconstructions from Patch-seq data, are normalized cortical depth (0.34 vs. 0.54 average for all Glutamatergic cells), Glutamatergic cells), dendrite total length (3357.7 vs. 4488.4 microns for all Glutamatergic cells), and stems (6.86 vs. 7.25 for all Glutamatergic cells). RNAseq 061-062 Subclass Zizhen Yao CS202002013_188 L4/5 IT L4 L4 L4/5 IT RNAseq 061-062, 065 - CS202002013_249