exemplar data of Cluster L4/5 IT_1 primary motor cortex glutamatergic neuron (Mmus) RNAseq 061-062 - CS202002013_188 RNAseq 061 - CS202002013_61 CS202002013_61 Nikolas Jorstad L4 IT L4/5 IT_1 PMID:34616066 {"cell_set_preferred_alias": "L4/5 IT_1", "original_label": "L4/5 IT_1", "cell_set_label": "RNAseq 061", "cell_set_accession": "CS202002013_61", "cell_set_aligned_alias": "L4 IT", "cell_set_additional_aliases": "", "cell_set_alias_assignee": "Zizhen Yao|Nikolas Jorstad", "cell_set_alias_citation": "PMID:34616066|PMID:34616062", "cell_set_structure": "primary motor cortex", "cell_set_ontology_tag": "UBERON:0001384", "species": "Mouse", "modality": "RNAseq", "taxonomy_id": "CCN202002013", "taxonomy_description": "Mouse MOp BICCN taxonomy using multiple RNA-Seq datasets ", "child_cell_set_accessions": "", "cell_type_card": "Cell Type", "cell_set_color": "#09CCC6"} L5 IT_C1 L4/5 IT_1 RNAseq 061 L4/5 IT_1 In Mouse primary motor cortex (CCN202002013), L4/5 IT_1 is a member of the L4/5 IT subclass. L4/5 IT_1 includes the Patch-seq and connectivity types L4 IT. The minimal set of markers required to distinguish this cell type from other cell types in the primary motor cortex is Cadps2 and Prr16 and Rorb (F-beta = 0.734), based on the NS Forest method of marker gene selection. L4/5 IT_1 cells represent 59.14% of all L4/5 IT cells collected in the snRNA-seq 10X v3 B dataset. L4/5 IT_1 cells have an average of 4365 genes detected, which is 311 less than the average for all L4/5 IT cells (4676). L4/5 IT_1 cells have an average firing rate of 30.14 Hz, compared to 26.01 Hz for all L4/5 IT cells recorded. Other distinguishing features include an input resistance of 183.23 MOhms (170.7 MOhms in all L4/5 IT cells), and upstroke:downstroke ratio of 4.2 (4.38 for all L4/5 IT cells). The most distinguishing morpholgical features of L4/5 IT_1, as determined by reconstructions from Patch-seq data, are normalized cortical depth (0.29 vs. 0.34 average for all L4/5 IT cells), dendrite total length (2265.8 vs. 3357.7 microns for all L4/5 IT cells), and stems (5.08 vs. 6.86 for all L4/5 IT cells). L4 IT Cell Type L4 IT Zizhen Yao PMID:34616062