exemplar data of Cluster L4/5 IT_2 primary motor cortex glutamatergic neuron (Mmus) RNAseq 061-062 - CS202002013_188 RNAseq 062 - CS202002013_62 Cell Type PMID:34616062 L4/5 IT L4/5 IT_2 CS202002013_62 Nikolas Jorstad {"cell_set_preferred_alias": "L4/5 IT_2", "original_label": "L4/5 IT_2", "cell_set_label": "RNAseq 062", "cell_set_accession": "CS202002013_62", "cell_set_aligned_alias": "L4/5 IT", "cell_set_additional_aliases": "", "cell_set_alias_assignee": "Zizhen Yao|Nikolas Jorstad", "cell_set_alias_citation": "PMID:34616066|PMID:34616062", "cell_set_structure": "primary motor cortex", "cell_set_ontology_tag": "UBERON:0001384", "species": "Mouse", "modality": "RNAseq", "taxonomy_id": "CCN202002013", "taxonomy_description": "Mouse MOp BICCN taxonomy using multiple RNA-Seq datasets ", "child_cell_set_accessions": "", "cell_type_card": "Cell Type", "cell_set_color": "#52B8AA"} L4/5 IT L4/5 IT Zizhen Yao L5 IT_C1 L4/5 IT_2 PMID:34616066 L4/5 IT_2 RNAseq 062 In Mouse primary motor cortex (CCN202002013), L4/5 IT_2 is a member of the L4/5 IT subclass. L4/5 IT_2 includes the Patch-seq and connectivity types L4/5 IT. The minimal set of markers required to distinguish this cell type from other cell types in the primary motor cortex is Col26a1 and Cpne4 and Il1rapl2 (F-beta = 0.707), based on the NS Forest method of marker gene selection. L4/5 IT_2 cells represent 40.86% of all L4/5 IT cells collected in the snRNA-seq 10X v3 B dataset. L4/5 IT_2 cells have an average of 5125 genes detected, which is 449 more than the average for all L4/5 IT cells (4676). L4/5 IT_2 cells have an average firing rate of 23.13 Hz, compared to 26.01 Hz for all L4/5 IT cells recorded. Other distinguishing features include an input resistance of 161.96 MOhms (170.7 MOhms in all L4/5 IT cells), and upstroke:downstroke ratio of 4.5 (4.38 for all L4/5 IT cells). The most distinguishing morpholgical features of L4/5 IT_2, as determined by reconstructions from Patch-seq data, are normalized cortical depth (0.37 vs. 0.34 average for all L4/5 IT cells), dendrite total length (3927.39 vs. 3357.7 microns for all L4/5 IT cells), and stems (7.78 vs. 6.86 for all L4/5 IT cells).