exemplar data of Cluster L5/6 NP CT primary motor cortex glutamatergic neuron (Mmus) RNAseq 074-080 - CS202002013_197 RNAseq 074 - CS202002013_74 L5/6 NP CT PMID:34616062 Zizhen Yao RNAseq 074 PMID:34616066 Cell Type L5 IT_3 CS202002013_74 L5/6 NP CT L5/6 NP CT {"cell_set_preferred_alias": "L5/6 NP CT", "original_label": "L5/6 NP CT", "cell_set_label": "RNAseq 074", "cell_set_accession": "CS202002013_74", "cell_set_aligned_alias": "L5 IT_3", "cell_set_additional_aliases": "", "cell_set_alias_assignee": "Zizhen Yao|Nikolas Jorstad", "cell_set_alias_citation": "PMID:34616066|PMID:34616062", "cell_set_structure": "primary motor cortex", "cell_set_ontology_tag": "UBERON:0001384", "species": "Mouse", "modality": "RNAseq", "taxonomy_id": "CCN202002013", "taxonomy_description": "Mouse MOp BICCN taxonomy using multiple RNA-Seq datasets ", "child_cell_set_accessions": "", "cell_type_card": "Cell Type", "cell_set_color": "#1AAB99"} L5 IT_3 In Mouse primary motor cortex (CCN202002013), L5/6 NP CT is a member of the L6 CT subclass. L5/6 NP CT includes the Patch-seq and connectivity types L5 IT_3. The minimal set of markers required to distinguish this cell type from other cell types in the primary motor cortex is Grp and Rxfp1 and Tshz2 (F-beta = 0.378), based on the NS Forest method of marker gene selection. L5/6 NP CT cells represent 0.82% of all L6 CT cells collected in the snRNA-seq 10X v3 B dataset. L5/6 NP CT cells have an average of 4336 genes detected, which is 521 less than the average for all L6 CT cells (4857). L5/6 NP CT cells have an average firing rate of 53.33 Hz, compared to 27.46 Hz for all L6 CT cells recorded. Other distinguishing features include an input resistance of 222.15 MOhms (204.84 MOhms in all L6 CT cells), and upstroke:downstroke ratio of 4.1 (3.41 for all L6 CT cells). The most distinguishing morpholgical features of L5/6 NP CT, as determined by reconstructions from Patch-seq data, are normalized cortical depth (0.49 vs. 0.8 average for all L6 CT cells), dendrite total length (3382.9 vs. 2884.47 microns for all L6 CT cells), and stems (5 vs. 6.97 for all L6 CT cells). Nikolas Jorstad