has part has part has_part autophagosome assembly autophagic vacuole formation GO:0000045 PAS formation autophagic vacuole assembly autophagosome formation The formation of a double membrane-bounded structure, the autophagosome, that occurs when a specialized membrane sac, called the isolation membrane, starts to enclose a portion of the cytoplasm. biological_process autophagosome biosynthesis Atg12 activating enzyme activity protein-phosphatidylethanolamide deconjugating activity Atg12 conjugating enzyme activity Atg12 ligase activity organelle assembly Atg1/ULK1 kinase complex assembly autophagosome organization