ends with results in assembly of heterochromatin chromatin organization cellular component assembly heterochromatin formation establishment of heterochromatin architecture establishment of heterochromatin architecture involved in chromatin silencing at pericentric region heterochromatin assembly involved in chromatin silencing at centromere outer repeat region chromatin-mediated silencing An epigenetic gene silencing mechanism in which chromatin is compacted into heterochromatin, resulting in a chromatin conformation refractory to transcription. This process starts with heterochromatin nucleation, its spreading, and ends with heterochromatin boundary formation. heterochromatin assembly involved in chromatin silencing heterochromatic silencing Reactome:R-HSA-5334118 transcriptional gene silencing biological_process heterochromatin maintenance establishment of heterochromatic silencing TGS heterochromatin formation involved in chromatin silencing GO:0031507 heterochromatin formation involved in chromatin silencing at pericentric region heterochromatin assembly involved in chromatin silencing at pericentric region establishment of chromatin silencing establishment of heterochromatin architecture involved in chromatin silencing at centromere outer repeat region chromatin silencing heterochromatin formation involved in chromatin silencing at centromere outer repeat region heterochromatin assembly heterochromatin boundary formation negative regulation of gene expression, epigenetic heterochromatin organization