has part realizes is about has_specified_input has_specified_output achieves_planned_objective has participant role of ribonucleic acid gene expression analyte role transcription profiling assay polyA RNA extraction sequence data RNA-seq assay JZ: should be inferred as 'DNA sequencing'. Will check in the future. An assay in which sequencing technology (e.g. Solexa/454) is used to generate RNA sequence, analyse the transcibed regions of the genome, and or to quantitate transcript abundance EFO_0002770 transcription profiling by high throughput sequencing PERSON: James Malone RNA-seq assay an assay that uses high-throughput sequencing technologies to sequence cDNA in order to get information about a sample's RNA content. RNA-Seq provides researchers with efficient ways to measure transcriptome data experimentally, allowing them to get information such as how different alleles of a gene are expressed, detect post-transcriptional mutations or identify gene fusions. WEB: http://en.wikipedia.org/wiki/RNA-Seq transcription profiling by high throughput sequencing transcription profiling identification objective specimen DNA sequencer sequencing assay metadata complete obi.owl