is about has_specified_input is_specified_output_of gene expression single fragment library RNA-seq assay count RPKM-reads per kilobase of transcript per million fragments mapped The definition of RPKM has been modified in October 2019 to reflect the possible uses as detailed in https://hbctraining.github.io/DGE_workshop/lessons/02_DGE_count_normalization.html and distinguish more clearly RPKM from measures such as TPM Alejandra Gonzalez Beltran Alejandra Gonzalez-Beltran Orlaith Burke Philippe Rocca-Serra RPKM RPKM is a kind of count which numbers the sequence reads found per kilobase of transcript reported to million of sequence reads. RPKM is a metric generated by ERANGE software tool as reported by Mortazi et al, in 2008. The metric has been enhanced and replaced by FPKM to better take into account splice variant. FKPM uses a statistical model to perform the computation. Recommended use: -RPKM measure may be used for gene count comparisons within a sample. -RPKM measure is NOT suited for between sample comparisons. -RPKM measure is NOT suited for Differential Expression analysis. http://www.nature.com/nmeth/journal/v5/n7/full/nmeth.1226.html pending final vetting