sticky_end_restriction_enzyme_cleavage_site five_prime_sticky_end_restriction_enzyme_cleavage_site five prime sticky end restriction enzyme cleavage site SO:0001975 kareneilbeck sequence Requested by Jackie Quinn. The sticky restriction sites are different from junctions because they include the sequence that is cut, inclusive of the five prime junction and the three prime junction. 2013-07-30T11:32:16Z A restriction enzyme recognition site that, when cleaved, results in 5 prime overhangs.