predicted_transcript unconfirmed_transcript sequence This is used for non-spliced EST clusters that have polyA features. This category has been specifically created for the ENCODE project to highlight regions that could indicate the presence of protein coding genes that require experimental validation, either by 5' RACE or RT-PCR to extend the transcripts, or by confirming expression of the putatively-encoded peptide with specific antibodies. to_be_experimentally_confirmed_transcript nicole TEC SO:0002139 2016-08-23T16:28:07Z