has part has_specified_input investigation clonal cDNA library imaging assay flow cytometry assay in live cell assay screening library normalization data transformation high-content screening Orlaith Burke Alejandra Gonzalez-Beltran Philippe Rocca-Serra adapted from: http://en.wikipedia.org/wiki/High-content_screening high content screening is a kind of investigation which uses a standardized cellular assays to test the effect of substances (RNAi or small molecules) held in libraries on a cellular phenotype. it relies on microscopy imaging and or flow-cytometry, robotic handling to ensure fast and high-throughput. high throughput screening hit selection between group comparison statistical test pending final vetting