binding (with molecule) CAD insertion into phospholipase binding site to lysosomal inner membrane CAD insertion into phospholipase binding site to lysosomal inner membrane is a subtype of binding: A process that CAD inserts and replaces the phospholipase binding site in the lysosomal inner membrane. Under normal conditions, lysosomal phospholipase A2 is bound through electrostatic charge interactions to the inner membranes of the lysosome. A cationic amphiphile, accumulates in the lysosome and inserts into the inner membrane decreasing or eliminating the anionic binding sites for the lipase. The lipase is no longer able to bind to its substrates and is potentially susceptible to degradation by lysosomal proteases.