has role 17β-estradiol 17-glucosiduronic acid cholestasis dependent chemical compound inhibitor role of BSEP bile acid transport esgtradiol‐17β‐ glucuronide [Cholestasis] Changes in Bsep localization are involved in E217G-induced impairment of bile flow and bile salt transport.a Mrp2 is required for E217G to induce its harmful effect. estradiol-17beta-glucuronide inhibited ATP-dependent taurocholate transport only in normal canalicular plasma membrane and in Bsep/Mrp2-coexpressing Sf9 cell vesicles, but not in Mrp2-deficient, indicating trans-inhibitionof Bsep only after its secretion into bile canaliculi by Mrp2.