cofactor metabolism cofactor biosynthesis GO:0051188 pathway The formation from simpler components of a cofactor, a non-protein chemical substance that is tightly bound to an enzyme and is required for catalysis. Cofactors can be considered 'helper molecules/ions' that assist in biochemical transformations. Coenzyme biosynthesis pathways, e.g coenzyme A biosynthesis are considered under this pathway-category. UPa:UPA00399