tRNA modification 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine-tRNA biosynthesis ms2io6A-tRNA biosynthesis 2-methylthio-cis-ribozeatin-tRNA biosynthesis GO:0002195 PMID:9620964 pathway UPa:UPA00729 Biosynthesis of 2-methylthio-N-6-isopentenyl adenosine (ms2i6A), a modified nucleoside present in position 37 (adjacent to and 3' of the anticodon) of tRNAs that read codons beginning with U except tRNA(i.v. Ser) in Escherichia coli. In Salmonella typhimurium, 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms2io6A; also referred to as 2-methylthio cis-ribozeatin) is found in tRNA, most likely in the species that have ms2i6A in E. coli. PMID:8253666