has function has role vaccinated in route immunizes against disease test in lab animal model melanoma Mus musculus vaccine in research role intramuscular route melanoma vaccine therapeutic vaccine function 1(SOCS1)-silenced dendritic cell vaccine https://github.com/vaccineontology/VO/issues/78 VIOLIN: https://violinet.org/vaxquery/vaccine_detail.php?c_vaccine_id=4071 Ibrahim Seleznev To obtain SOCS1-silenced DCs, DCs derived from mouse bone marrow cells ex vivo were induced to differentiation in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4, and then transduced with Len-SOCS1-shRNA or control Len-GFP lentiviruses. The SOCS1-silenced DCs were loaded by TRP2 peptide to prepare the DC vaccine, which was induced to mature by LPS. The DCs were analyzed by flow cytometry (FCM) for surface expressions of MHCII and CD86 and by real-time PCR for the expressions of SOCS1, IL-10 as well as IL-12p40. B16 or IL-10-silenced B16 (IL-10(-/-);) cells were inoculated into C57BL/6 mice. 1(SOCS1)-silenced dendritic cell (DC) Vaccine PMID:23643168 cancer vaccine 4071 Jie Zheng Eliyas Asfaw Oliver He therapeutic cancer vaccine