has role immunizes against disease immunizes recipient immunizes against pathogen test in lab animal model brucellosis Mus musculus Brucella melitensis Bos taurus vaccine in research role live attenuated Brucella melitensis vaccine cattle vaccine live attenuated Brucella melitensis purE deletion vaccine 2026-10-25T08:06:33Z 12024 VaxAgent Live attenuated Brucella melitensis vaccine using live attenuated Brucella melitensis strain 16M with pure gene deletion (delta pure201). Delta pure201 mutant showed significantly reduced bacterial counts in spleen, liver, and lungs compared to wild-type 16M. Mice infected with delta pure201 showed mild transient splenomegaly and no serum interleukin-6. VIOLIN: https://violinet.org/vaxquery/vaccine_detail.php?c_vaccine_id=12024 vaccine PMID:8675325 Jie Zheng live attenuated vaccine role