has role immunizes against disease immunizes against pathogen test in lab animal model brucellosis Mus musculus Brucella abortus vaccine in research role live attenuated Brucella abortus vaccine live attenuated Brucella abortus INTA2 vaccine 2026-10-25T08:06:33Z PMID:18565697 VIOLIN: https://violinet.org/vaxquery/vaccine_detail.php?c_vaccine_id=12062 PMID:12079742 12062 Jie Zheng VaxAgent Live attenuated Brucella abortus vaccine using live attenuated double mutant strain with bp26 replaced by luciferase gene and partial deletion of bmp18, lacking drug resistance markers. Inta2 showed significantly reduced bacterial load in spleens during early infection and no splenomegaly, indicating reduced residual virulence. Inta2 induced o-side antibodies but did not express bp26, enabling differentiation of vaccinated animals from those infected with field strains by measuring anti-bp26 antibodies via agglutination test or ELISA. vaccine live attenuated vaccine role