has role immunizes against disease immunizes against pathogen test in lab animal model brucellosis Mus musculus Brucella ovis vaccine in research role live attenuated Brucella ovis vaccine live attenuated Brucella ovis pyruvate phosphate dikinase deletion vaccine 2026-10-25T08:06:33Z VaxAgent PMID:32795361 Live attenuated Brucella ovis vaccine using live attenuated mutant strains with deletion of the ppdk gene impairing growth and virulence. Ppdk mutant showed impaired growth in vitro, inability to multiply in infected cells, and an unusual attenuation pattern in mice with initial higher spleen colonization followed by marked decrease, indicating attenuation and potential as a vaccine candidate. VIOLIN: https://violinet.org/vaxquery/vaccine_detail.php?c_vaccine_id=12388 vaccine Jie Zheng 12388 live attenuated vaccine role