has role immunizes against disease immunizes against pathogen test in lab animal model brucellosis Mus musculus Brucella abortus vaccine in research role recombinant vector vaccine role Brucella abortus recombinant vector vaccine live attenuated Brucella abortus vaccine live attenuated Brucella abortus BAB1_0278 Lactococcus lactis pNZ8148-Usp45 vector vaccine 2026-10-25T08:06:33Z L. lactis-pNZ8148-BAB1-0278-Usp45 vaccine VaxAgent Jie Zheng 12458 vaccine VIOLIN: https://violinet.org/vaxquery/vaccine_detail.php?c_vaccine_id=12458 Live attenuated Brucella abortus vaccine using the BAB1-0278 gene was cloned into the pnz8148 vector, transformed into L. Lactis, and the target protein was extracted and confirmed by SDS-PAGE and western blot. Vaccinated mice showed significantly higher total igg, igg1, and igg2a levels compared to PBS controls. Vaccine induced both humoral (igg subclasses) and cellular immunity (increased cytokines ifn-γ, tnfα, il-4, il-10), along with enhanced lymphocyte proliferation and modulation of innate immune markers. PMID:37866552 live attenuated vaccine role