has role immunizes against disease immunizes against pathogen test in lab animal model brucellosis Mus musculus Brucella melitensis vaccine in research role live attenuated Brucella melitensis vaccine live attenuated Brucella melitensis purD deletion vaccine 2026-10-25T08:06:33Z VaxAgent VIOLIN: https://violinet.org/vaxquery/vaccine_detail.php?c_vaccine_id=12518 PMID:41477197 Jie Zheng 12518 vaccine Live attenuated Brucella melitensis vaccine using A live attenuated strain with deletion of the purd gene, impairing purine biosynthesis. Purd mutant showed impaired growth, increased sensitivity to detergent, reduced membrane permeability, altered lipid biosynthesis, decreased ability to invade and survive in host cells, and reduced colonization and pathology in mice. live attenuated vaccine role