has role protective antigen role protective antigen Eimeria tenella protein TA4 Oliver He Protegen ID: 461; NCBI Protein GI: 84783094; NCBITaxon: 5802 protein Allen Xiang The immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella, including route, dose, time of immunization and age of primary immunization of chicken, was optimized. results illustrated that 25 microg was the optimal dose and intramuscular injection was the most effective route to induce protective immunity in Chinese Yellow chickens [PubMed: 19019543].