controlled serum-free condition Media 199 2023-04-06T12:56:12Z M199 M199 media M199 medium Medium 199 PMID:31283468 This is any experimental condition in which the main influencing factor is M199 medium, which is useful across a wide range of species and applications but is particularly suitable for the culturing of non-transformed cells. M199 medium ingredients may include Earle’s salts and a bicarbonate/carbonic acid buffering system for the maintenance of pH in a CO2 incubator or Hank’s salts with salt solutions designed for atmospheric equilibrium. XCO:0001009 experimental_condition_ontology slaulede