immortal mouse embryo-derived fibroblast cell line cell Sufu-KO-LIGHT cell JX, YH WEB: https://www.google.com/patents/WO2013192301A1?cl=en Sufu-KO-LIGHT cells were derived from Sufu knockout mouse embryonic fibroblasts according to the method of Chen et al. [Genes Dev. 2009;23(16): 1910-28.] The cells were co-transfected with the zeocin resistance vector (pVgRXR, Invitrogen) and a firefly luciferase reporter driven by eight Gli binding sites and a γ-crystallin basal promoter (8XGHBS-FL). Sufu-KO-LIGHT lacks Sufu and exhibits constitutive Hh target gene expression in response to endogenous Gli activators. PubChem AID: 588413